NOVEL DNA-BINDING PROTEIN FROM DROSOPHILA EMBRYOS IDENTIFIED BY BINDING-SITE SELECTION

The DNA sequence C/AGAGCGC/AGA, related to binding sites for GAF and Z este transcription factors, was selected from a pool of degenerate PCR fragments for binding to the cytoplasmic protein of Drosophila prebla stoderm embryos, identical DNA binding activity was also detected in e mbryonic nuclei, Based on several criteria, such as size, intracellula r distribution, sensitivity to ATP and protein kinase inhibitor 6-DMAP , kinetics during development and lack of cross-reaction with rabbit a nti-GAF serum, protein recognizing selected sequence was shown to diff er from either Zeste or GAF.