Atypical mycobacteria are usually found in ground and water. Their dis
tribution are different according to species and regions. The aim of t
his study was to assess the presence of atypical mycobacteria in Lorra
ine : northe-east French region, 81 water samples and 36 soil samples
were collected from various sites close to Nancy, All samples were dec
ontaminated with Lauryl sulfate Na, grown on Loewsenstein Jensen cultu
re and incubated at 30, 37 and 40 degrees C. The results indicate a hi
gh contamination rate by non-acid fast bacteria. 33 samples were disca
rded. Atypical mycobacteria were isolated from 42 water samples (59 %)
and 9 soil samples (69 %). The most important species were saprophyti
c : M. gordonae, M. fortuitum. Among the opportunistic species, M. xen
opi was isolated from one water sample, M. kansasii and M. marinum wer
e isolated from 6 water samples, MAC were almost always present in the
ground samples.