ANCHORING OF C-MYC ON NUCLEAR MATRIX PROTEINS IN-PROCESS OF MOUSE THYMIC T-LYMPHOCYTE PROLIFERATION INDUCED BY CONA

Isolation and characterization of functional nuclear matrix proteins i nvolved in DNA anchoring and gene expression is one of the major subje cts of current nuclear matrix research. Southwestern blotting (DNA-pro tein hybridization) was applied to studying the anchoring of c-myc on the nuclear matrix proteins in mouse thymic T lymphocytes. The results showed that c-myc bound to the lamin, p34 and p36 nuclear matrix prot eins specifically. In the process of mouse thymic PNA(-)T lymphocytes proliferation induced by ConA, the anchoring of c-myc on p34 and p36 n uclear matrix proteins changed dynamically.