MOLECULAR MARKERS OF NUCLEAR RESTORATION GENE RF1 IN SUNFLOWER USING BULKED SEGREGANT ANALYSIS-RAPD

Restoration of cytoplasmic male sterility (CMS) in sunflower was demon strated to be controlled by polygenes by analysing 982 effective cross es among 109 self-crossed lines and 16 CMS lines. Two self-crossed lin es and one CMS line with distinct genotypes were applied to creation o f segregating populations for DNA bulks of the target gene Rf1. Bulked DNA was prepared in order to investigate single gene Rf1 and its gene marker among polygenic characters at the same genetic background, Usi ng 80 10-mer operon primers, 620 RAPD reactions were carried out betwe en fertile and sterile DNA bulks. In about 800 loci, primary results s howed that 8 were related to the restoration genes. Furthermore, 2 wer e confirmed as RAPD markers for gene Rf1 by examining 9 maintenance an d 7 restoration lines. This method is the improvement for bulked segre gant analysis([1]) with which markers of single gene of target on be i dentified rapidly among polygenic characters.