QUANTITATION OF INTRACELLULAR ZIDOVUDINE PHOSPHATES BY USE OF COMBINED CARTRIDGE RADIOIMMUNOASSAY METHODOLOGY

Authors
ROBBINS BL WAIBEL BH FRIDLAND A
Citation
Bl. Robbins et al., QUANTITATION OF INTRACELLULAR ZIDOVUDINE PHOSPHATES BY USE OF COMBINED CARTRIDGE RADIOIMMUNOASSAY METHODOLOGY, Antimicrobial agents and chemotherapy, 40(11), 1996, pp. 2651-2654
Citations number
23
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
40
Issue
11
Year of publication
1996
Pages
2651 - 2654
Database
ISI
SICI code
0066-4804(1996)40:11<2651:QOIZPB>2.0.ZU;2-7
Abstract
This report describes the development of a potentially clinical method to measure the cellular metabolites of zidovudine (ZDV) in patients r eceiving the drug, This new method combines the use of Sep-Pak cartrid ges to separate ZDV phosphates with radioimmunoassaying to quantitate ZDV, The detection limit is 0.02 pmol/10(6) cells, and this assay can measure a wide range of intracellular drug concentrations, The use of the cartridge-radioimmunoassay methodology should prove very useful fo r in vivo cellular pharmacokinetic studies of ZDV.