ENGINEERING THE SELECTIVITY OF ALIPHATIC C-H BOND OXIDATION CATALYZEDBY CYTOCHROME P450CAM

Authors
JONES NE ENGLAND PA ROUCH DA WONG LL
Citation
Ne. Jones et al., ENGINEERING THE SELECTIVITY OF ALIPHATIC C-H BOND OXIDATION CATALYZEDBY CYTOCHROME P450CAM, Chemical communications, (21), 1996, pp. 2413-2414
Citations number
12
Categorie Soggetti
Chemistry
Journal title
Chemical communications → ACNP
ISSN journal
13597345
Issue
21
Year of publication
1996
Pages
2413 - 2414
Database
ISI
SICI code
1359-7345(1996):21<2413:ETSOAC>2.0.ZU;2-R
Abstract
The regioselectivity of the catalytic hydroxylation of phenylcyclohexa ne 1 by cytochrome P450cam can be altered dramatically by site-directe d mutagenesis: the Y96F single site mutant gives 81% cis-3-phenylcyclo hexanol 2, with 22% enantiomeric excess (ee), while the Y96F-V247A dou ble mutant gives 97% 2 with 42% ee and the Y96F-V247L double mutant gi ves 83% trans-4-phenylcyclohexanol 4.