Ne. Jones et al., ENGINEERING THE SELECTIVITY OF ALIPHATIC C-H BOND OXIDATION CATALYZEDBY CYTOCHROME P450CAM, Chemical communications, (21), 1996, pp. 2413-2414
The regioselectivity of the catalytic hydroxylation of phenylcyclohexa
ne 1 by cytochrome P450cam can be altered dramatically by site-directe
d mutagenesis: the Y96F single site mutant gives 81% cis-3-phenylcyclo
hexanol 2, with 22% enantiomeric excess (ee), while the Y96F-V247A dou
ble mutant gives 97% 2 with 42% ee and the Y96F-V247L double mutant gi
ves 83% trans-4-phenylcyclohexanol 4.