INDUCTION OF A PUTATIVE SERINE-PROTEASE TRANSCRIPT IN IMMUNE CHALLENGED DROSOPHILA

In an effort to identify serine proteases involved in the insect's imm une response, we used a degenerate PCR approach to amplify putative se rine protease gene fragments in Drosophila, Sequencing of the cloned P CR products identified one serine protease previously isolated in D. m elanogaster (SER1/SER2), as well as two novel putative serine protease gene fragments (SP2, SP3). The involvement of the corresponding genes in the immune response was examined by analyzing their expression in larval mRNA following both parasitic and bacterial exposures. The over expression of one of the serine proteases-related mRNAs in immune chal lenged larvae suggests its involvement in the Drosophila immune respon se. Copyright (C) 1996 Published by Elsevier Science Ltd.