LIVER-GLYCOGEN IN FASTED RAT LIVERS DOES NOT IMPROVE OUTCOME OF LIVER-TRANSPLANTATION

Controversy exists over how the nutritional condition of the donor liv er affects transplant outcome. Some studies suggest that livers from f asted animals (liver glycogen-depleted) are more readily injured than livers from fed animals. Our previous study suggested the opposite, i. e., livers from donors fasted for 4 days were significantly more viab le on orthotopic liver transplantation. Fasting may decrease the sensi tivity of the liver to an inflammatory response or block Kupffer cell activation following transplantation. Thus, long-term fasting may be b eneficial for reasons unrelated to liver glycogen content. In this stu dy we attempted to separate out the roles of fasting and liver glycoge n in liver transplant outcome by fasting donors for 2 days and then fe eding them only glucose to elevate liver glycogen. Rats (Brown Norway) were fed (standard diet), fasted (4 days), or fasted 2 days and then fed glucose (in water) for 2 days. Livers were preserved for either 30 or 44 h in UW solution and transplanted. Four-day fasting of the dono r improved the survival rate in liver transplantation (50 %-100 % in 3 0-h cold storage, 29 %-83 % in 44-h cold storage). However, feeding gl ucose for 2 days to fasted animals caused a decrease in survival in th is series of transplants (40 % in 30-h cold storage, 0 % in 44-h cold storage). In the glucose-fed group, liver glycogen was 240 % of that i n the control group. This suggests that the presence of a high concent ration of liver glycogen is not beneficial to the preserved and transp lanted rat liver.