BIOFLUORESCENT SUBSTANCES FOR SAPID ASSESSMENT OF TOXICANTS ON INVERTEBRATES

The recently developed 1 h fluorescent test with Daphnia magna was fur ther evaluated. The assay is based on the in vivo observation of enzym atic activity using a fluorogenic substrate that, after cleavage by th e corresponding enzyme, releases a fluorescent dye. Influences interfe ring with the test evaluation were determined and eliminated. It was i nvestigated as to whether new fluorescent tests can be developed for C eriodaphnia dubia and Hyalella azteca. Also, other fluorescent substra tes were tested for their applicability as fluorogenic substrate in co mbination with D. magna, C. dubia, and H. azteca. (C) 1996 by John Wil ey & Sons, Inc.