The highest activity of extracellular lipases was obtained after 24 h
of cultivation of R. miehei NH in the medium with 0.5% beef tallow and
medium working volume of 100 mi (2302U/ug of protein). For yeast afte
r 36 h of cultivation for Y.lipolytica 2, in 50 mi medium with 0.5% be
ef tallow (1796 U/ug of protein). Lipases activity increased by loweri
ng the working volume of medium and decreasing the glucose content.