SIMULTANEOUS MIXTURE ANALYSIS USING A DYNAMIC MICROBIAL SENSOR COMBINED WITH CHEMOMETRICS

A biosensor consisting of immobilized microbial cells and an oxygen el ectrode was used in a flow-through system as a microbial sensor now in jection analyzer (FIA), For different organic analytes, the metabolism of vital cells provides individual time-resolved responses with disti nct time-dependent amplitudes, Chemometrical data analysis revealed th at the individual responses are additive and depend linearly on single analyte concentrations. Based on these observations, simultaneous mul ticomponent analysis of organic mixtures was carried out in the FIA's time domain with analytical errors of less than 10%, For mixture analy sis and monitoring in processes like enzymatic conversions, the descri bed microbial sensor FIA (''dynamic microbial sensor'') offers an alte rnative to expensive analytical equipment.