AN ULTRASENSITIVE COLORIMETRIC METHOD FOR THE ASSAY OF ENDO-1,4-BETA-D-GLUCANASE

Authors
SESTAK S FARKAS V
Citation
S. Sestak et V. Farkas, AN ULTRASENSITIVE COLORIMETRIC METHOD FOR THE ASSAY OF ENDO-1,4-BETA-D-GLUCANASE, Biotechnology techniques, 10(10), 1996, pp. 731-734
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
10
Year of publication
1996
Pages
731 - 734
Database
ISI
SICI code
0951-208X(1996)10:10<731:AUCMFT>2.0.ZU;2-T
Abstract
A colorimetric method for the assay of endo-1,4-beta-D-glucanase from fungal cellulolytic systems which utilizes xyloglucan as substrate is described. The substrate forms a blue-green complex with I-2 which is lost upon its breakdown with the enzyme. The new assay is a simple one -step procedure and its sensitivity is comparable with that of viscome try.