TECHNICAL ASPECTS OF EXTRACTIVE PURIFICATION OF PENICILLIN FERMENTATION BROTH BY AQUEOUS 2-PHASE PARTITIONING

A new attempt was made to reform the original penicillin recovery tech nology, and fundamental data were provided for further scale-up. The v olume of fermentation broth processed was 1000 mL, the mass of penicil lin crystal obtained was 7.228 g with an average purity of 84.15%, the overall recovery ratio of aqueous two-phase partitioning (ATPP) techn ology was 76.56%, and the entire technical process is described. Compa red to the original organic solvent extraction, ATPP technology can ex tract penicillin from whole fermentation broth directly. The pH should be adjusted only once and the activity of penicillin can be improved. The extraction steps are decreased from three times to one, and the e xtraction technology is integrated. A new field is opened up by this p owerful ATPP technology.