BIOTIN-LABELED ANTIGEN - A NOVEL-APPROACH FOR DETECTION OF PUUMALA VIRUS-SPECIFIC IGM

Authors
IVANOV A VAPALAHTI O LANKINEN H TKACHENKO E VAHERI A NIKLASSON B LUNDKVIST A
Citation
A. Ivanov et al., BIOTIN-LABELED ANTIGEN - A NOVEL-APPROACH FOR DETECTION OF PUUMALA VIRUS-SPECIFIC IGM, Journal of virological methods, 62(1), 1996, pp. 87-92
Citations number
23
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
62
Issue
1
Year of publication
1996
Pages
87 - 92
Database
ISI
SICI code
0166-0934(1996)62:1<87:BA-ANF>2.0.ZU;2-Q
Abstract
A novel enzyme-linked immunosorbent assay, BLA IgM-ELISA, based on bac ulovirus-expressed Puumala (PUU) virus nucleocapsid protein, was devel oped for rapid diagnosis of nephropathia epidemica. The recombinant an tigen (bac-PUU-N) was purified to homogeneity by HPLC and conjugated t o biotin. The biotin-streptavidin system, in combination with the mu-c apture technique, rendered the BLA IgM-ELISA a sensitivity similar to or higher than that of PUU virus IgM mu-capture ELISA based on native antigen. The assay was shown to be highly specific when evaluated usin g a panel of 160 patient and control sera.