S. Nagase et al., DECREASED SERUM ANTIOXIDANT ACTIVITY OF HEMODIALYSIS-PATIENTS DEMONSTRATED BY METHYLGUANIDINE SYNTHESIS AND MICROSOMAL LIPID-PEROXIDATION, Nephron, 74(3), 1996, pp. 555-560
This study aims to raise the possibility of methylguanidine, a peroxid
ative product of creatinine, as a measure of the peroxidative state. A
s a known standard, we measured the inhibitory effect of uremic serum
on the NADPH-dependent microsomal lipid peroxidation. This is an estab
lished method for evaluating the peroxidative state and is compared to
the effect of uremic serum on methylguanidine synthesis. The study sh
ows decreased serum antioxidant activity in hemodialysis patients by b
oth methods, though there is no correlation between them. These result
s support the use of methylguanidine as a peroxidative marker and sugg
est a difference in the reactive oxygen species involved in the reacti
ons of methylguanidine synthesis and microsomal lipid peroxidation.