CADMIUM(II) BINDING TO RECOMBINANT MOUSE ZN-3-BETA-METALLOTHIONEIN-1

Authors
CAPDEVILA M ROMERO N COLS N ATRIAN S STILLMAN MJ GONZALEZDUARTE R GONZALEZDUARTE P
Citation
M. Capdevila et al., CADMIUM(II) BINDING TO RECOMBINANT MOUSE ZN-3-BETA-METALLOTHIONEIN-1, Anales de quimica, 92(4), 1996, pp. 199-201
Citations number
21
Categorie Soggetti
Chemistry
Journal title
Anales de quimica → ACNP
ISSN journal
11302283
Volume
92
Issue
4
Year of publication
1996
Pages
199 - 201
Database
ISI
SICI code
1130-2283(1996)92:4<199:CBTRMZ>2.0.ZU;2-N
Abstract
The genetic engineering techniques are coupled with spectroscopic anal ysis to characterize the metal-binding properties of the beta fragment of mouse metallothionein 1. UV absorption and CD titrations of recomb inant Zn-3-beta MT with CdCl2 show formation of a Cd-3-beta MT species , now reported for the first time. In addition, further Cd(II) induces refolding of the protein to form a Cd-9-beta MT species with a superc oiled 3D structure that exhibits a unique CD spectrum.