N. Bouaicha et al., DETECTION OF CYANOBACTERIAL TOXINS (MICROCYSTINS) IN CELL-EXTRACTS BYMICELLAR ELECTROKINETIC CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 685(1), 1996, pp. 53-57
Citations number
21
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A micellar electrokinetic chromatography (MEKC) method with UV detecti
on is described for the rapid and efficient separation of three microc
ystins: microcystin-YR (MCYST-YR), microcystin-LR (MCYST-LR) and micro
cystin-RR (MCYST-RR). A detection limit of 7.5 pg for each toxin was a
chieved. The UV intensities of these toxins measured at 200 nm showed
good linearity in the range 7.5-150 pg. The production of MCYST-LR in
three cultured strains of cyanobacteria, namely Microcystis aeruginosa
strain IP7806, Microcystis aeruginosa strain IP7813 and Oscillatoria
agardhii strain IP7805, was evaluated.