DETECTION OF CYANOBACTERIAL TOXINS (MICROCYSTINS) IN CELL-EXTRACTS BYMICELLAR ELECTROKINETIC CHROMATOGRAPHY

Authors
BOUAICHA N RIVASSEAU C HENNION MC SANDRA P
Citation
N. Bouaicha et al., DETECTION OF CYANOBACTERIAL TOXINS (MICROCYSTINS) IN CELL-EXTRACTS BYMICELLAR ELECTROKINETIC CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 685(1), 1996, pp. 53-57
Citations number
21
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
685
Issue
1
Year of publication
1996
Pages
53 - 57
Database
ISI
SICI code
Abstract
A micellar electrokinetic chromatography (MEKC) method with UV detecti on is described for the rapid and efficient separation of three microc ystins: microcystin-YR (MCYST-YR), microcystin-LR (MCYST-LR) and micro cystin-RR (MCYST-RR). A detection limit of 7.5 pg for each toxin was a chieved. The UV intensities of these toxins measured at 200 nm showed good linearity in the range 7.5-150 pg. The production of MCYST-LR in three cultured strains of cyanobacteria, namely Microcystis aeruginosa strain IP7806, Microcystis aeruginosa strain IP7813 and Oscillatoria agardhii strain IP7805, was evaluated.