TOWARD, A NEW METHOD TO IN-SITU STUDY OF APOPTOSIS AND ITS RELATIONS WITH CELL-CYCLE

Citation
F. Wallet et al., TOWARD, A NEW METHOD TO IN-SITU STUDY OF APOPTOSIS AND ITS RELATIONS WITH CELL-CYCLE, Cytometry, 25(3), 1996, pp. 263-270
Citations number
37
Categorie Soggetti
Cell Biology","Biochemical Research Methods
Journal title
ISSN journal
01964763
Volume
25
Issue
3
Year of publication
1996
Pages
263 - 270
Database
ISI
SICI code
0196-4763(1996)25:3<263:TANMTI>2.0.ZU;2-5
Abstract
Apoptosis or programmed cell death plays a key role in many biological processes particularly in oncology. The detection of apoptotic cells is crucial for the study of the phenomenon itself and its relation wit h the proliferative cell cycle. A new method to detect apoptosis in si tu in Feulgen stained cells was developed, based on multiparametric an alysis (fratorial and decisional analysis), using 15 densitometric and textural parameters measured on a SAMBA 200 cell image processor, Six reference files corresponding to G1, S, and G2 phases and to apoptoti c cells derived from these cell cycle phases were constructed, The pro jection of these files in the factorial principal plane formed distinc t clusters. Using the decisional discriminant analysis, it was possibl e to ascertain the state (apoptosis or proliferative ) and the phase f or each cell of a population, The correct classification rate of this analysis was 0.9962, Determining the cell cycle phase from which each apoptotic cell comes, we are able to study the relation between apopto sis and the proliferative cell cycle, Moreover, the detection in situ allows us to study cell-cell interactions. (C) 1996 Wiley-Liss, Inc.