Apoptosis or programmed cell death plays a key role in many biological
processes particularly in oncology. The detection of apoptotic cells
is crucial for the study of the phenomenon itself and its relation wit
h the proliferative cell cycle. A new method to detect apoptosis in si
tu in Feulgen stained cells was developed, based on multiparametric an
alysis (fratorial and decisional analysis), using 15 densitometric and
textural parameters measured on a SAMBA 200 cell image processor, Six
reference files corresponding to G1, S, and G2 phases and to apoptoti
c cells derived from these cell cycle phases were constructed, The pro
jection of these files in the factorial principal plane formed distinc
t clusters. Using the decisional discriminant analysis, it was possibl
e to ascertain the state (apoptosis or proliferative ) and the phase f
or each cell of a population, The correct classification rate of this
analysis was 0.9962, Determining the cell cycle phase from which each
apoptotic cell comes, we are able to study the relation between apopto
sis and the proliferative cell cycle, Moreover, the detection in situ
allows us to study cell-cell interactions. (C) 1996 Wiley-Liss, Inc.