IDENTIFYING AND MAPPING A NEW GENE FOR BACTERIAL-BLIGHT RESISTANCE INRICE-BASED ON RFLP MARKERS

Bacterial blight, caused by Xanthomonas oryzae pv. oryzae, is one of t he most serious diseases of rice worldwide. We previously identified a rice cultivar, Zhachanglong, from Yunnan Province in southwest China that showed resistance to all 10 X. oryzae pv. oryzae strains tested. The objectives of the current study were to assess the identity of the gene in Zhachanglong and to determine the chromosomal location by res triction fragment length polymorphism (RFLP) mapping. We found that th e resistance of Zhachanglong to bacterial blight is controlled by a do minant gene that is not allelic to Xa1, Xa2, Xa4, or Xa14. The new gen e is linked to the Xa4 locus, with a recombination frequency of 13.3 /- 4.5%. RFLP analysis resolved this gene to the end of the short arm of chromosome 11 in the rice linkage map, confirming the distinctness of this gene from all the known genes for bacterial blight resistance. We also found that this new gene is resistant to a broad range of X. oryzae pv. oryzae strains. We tentatively designated this gene as Xa22 (t).