REGIONS IN THE Z5 MATING GENE OF SCHIZOPHYLLUM-COMMUNE INVOLVED IN Y-Z BINDING AND RECOGNITION

The A alpha mating locus of the woodrotting fungus Schizophyllum commu ne encodes two multiallelic genes, Y and Z, which regulate the A-pathw ay of development. The Y alleles contain a homeobox, suggesting that t he Y proteins may be DNA-binding regulatory proteins. During mating, d evelopment is induced when Y from one mating partner interacts with Z from the other mating partner; self combinations of Y and Z are inacti ve. Two-hybrid analyses indicate that nonself combinations of Y and Z form heteromultimers and self combinations do not. To understand Y-Z b inding and self-nonself recognition further we used mutagenesis and ch imeras to identify regions in one allele of Z(Z5) that are involved in these processes. Here we report the results, which broadly define reg ions in Z5 that are essential for activity, Y-Z binding and Z5 allelic specificity.