HISTOCHEMICAL DETECTION OF EXPRESSION OF BINDING-SITES FOR LABELED HYALURONIC-ACID AND CARRIER-IMMOBILIZED SYNTHETIC (HISTO-BLOOD GROUP TRISACCHARIDES) OR BIOCHEMICALLY PURIFIED (GANGLIOSIDE GM(1)) GLYCOLIGANDS IN NASAL POLYPS AND OTHER HUMAN LESIONS INCLUDING NEOPLASMS

This study is intended to demonstrate the versatility and feasibility of custom-made oligosaccharide-exposing neoglycoconjugates including h isto-blood group epitopes in various human lesions, including nasal po lyps. The binding of the biotinylated probes was determined on formali n-fixed paraffin-embedded sections from archive materials. The general aspects of our results may be interpreted as follows: the neoglycocon jugates used here can readily detect differences in the ability of cel ls to bind glycan residues in tissue sections, thereby enabling the ex tent of the binding capacity of various types of human lesions to be c ompared. Furthermore, the reactivity to glycan may reflect characteris tics of the cells and their environment. The investigation into pathol ogical disorders with respect to the binding capacity of these carrier -immobilized mono- or oligosaccharide structures derived from custom-m ade synthesis or biochemical purification is based on the prospect of translating progress in this field into the establishment of potential ly beneficial procedures for medical diagnosis and pathological classi fication.