THE EFFECTS OF INDOMETHACIN, NDGA, ALLOPURINOL AND SUPEROXIDE-DISMUTASE ON PROSTAGLANDIN E(2) AND LEUKOTRIENE C-4 LEVELS AFTER MESENTERIC ISCHEMIA-REPERFUSION INJURY

In this study, the changes of arachidonic acid metabolites after an is chemia-reperfusion (I/R) period are investigated. The cyclooxygenase a nd lipoxygenase metabolites were found to be significantly increased a fter a 45 min period of ischemia followed by 5 min of reperfusion. Pro staglandin E(2) (PGE(2))- and leukotriene C-4 (LTC(4))-like activities did not change in the ischemic period, but they both increased after reperfusion. A cyclooxygenase inhibitor indomethacin and lipoxygenase inhibitor nordehydroguaretic acid (NDGA) decreased PGE(2)- and LTC(4)- like activities, respectively, while allopurinol and superoxide dismut ase (SOD) decreased both activities. According to our results, it can be assumed that free oxygen radicals are responsible for the elevation of PGE(2)- and LTC(4)-like activities and both of these arachidonic a cid metabolites and free oxygen radicals are the main necrotizing agen ts in ischemia-reperfusion induced damage.