Two types of anaerobic sludge were analyzed for ECP (extracellular pol
ymers) content under five extraction conditions. Results showed that E
DTA was more effective than formaldehyde as an extractant. Increase of
temperature and addition caustic also enhanced the extraction. The ra
tio between carbohydrate and protein fractions of ECP for both acetate
- and benzoate-degrading sludge was 0.16-0.18. The former sludge had o
nly 40-45% of ECP as in the latter sludge.