STABILITY OF ALPHA-CHYMOTRYPSIN CONJUGATED WITH POLY(ETHYLENE GLYCOLS) AND PROXANOLS AT HIGH-TEMPERATURE AND IN WATER-COSOLVENT MIXTURES

Authors
MOZHAEV VV KUDRYASHOVA EV EFREMOVA NV TOPCHIEVA IN
Citation
Vv. Mozhaev et al., STABILITY OF ALPHA-CHYMOTRYPSIN CONJUGATED WITH POLY(ETHYLENE GLYCOLS) AND PROXANOLS AT HIGH-TEMPERATURE AND IN WATER-COSOLVENT MIXTURES, Biotechnology techniques, 10(11), 1996, pp. 849-854
Citations number
24
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
11
Year of publication
1996
Pages
849 - 854
Database
ISI
SICI code
0951-208X(1996)10:11<849:SOACWP>2.0.ZU;2-6
Abstract
alpha-Chymotrypsin has been modified with poly(ethylene glycols) and p roxanols, block-copolymers of poly(propylene oxide) and poly(ethylene oxide). These conjugates were several-fold more thermostable and showe d high catalytic activity at elevated concentrations of water-miscible organic cosolvents (alcohols and dimethyl sulfoxide) which caused ina ctivation of free (non-modified) alpha-chymotrypsin.