IMPROVED MTT(3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHENYL TETRAZOLIUM BROMIDE) ASSAY FOR THE MEASUREMENT OF VIABLE ANIMAL-CELL NUMBER IN POROUS CELLULOSE CARRIERS

Authors
NOMURA N SAIJO K KATO M WANG PC OHNO T MATSUMURA M
Citation
N. Nomura et al., IMPROVED MTT(3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHENYL TETRAZOLIUM BROMIDE) ASSAY FOR THE MEASUREMENT OF VIABLE ANIMAL-CELL NUMBER IN POROUS CELLULOSE CARRIERS, Biotechnology techniques, 10(11), 1996, pp. 883-888
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
11
Year of publication
1996
Pages
883 - 888
Database
ISI
SICI code
0951-208X(1996)10:11<883:IMTB>2.0.ZU;2-X
Abstract
A method to measure cell concentration in porous cellulose carriers wa s developed. Results of the comparison of 5 colorimetric assays showed that MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromid e) assay by the solubilization of the formazan with DMSO(dimethyl sulf oxide) was the most suitable method for the measurement of cell concen tration in porous cellulose carriers. The growth of CHO-K1(Chinese ham ster ovary)-immobilized in different types of carrier was evaluated.