CHEMILUMINESCENCE OF THE TUMOR-ASSOCIATED MACROPHAGES AND THEIR ABILITY TO BIND AND LYSE AUTOLOGOUS TUMOR-CELLS

The investigation of the interrelationship between the capacity for ta rget binding, cytolysis and production of reactive oxygen intermediate s (ROI) by tumor-associated macrophages (TAM) was carried out, TAM abi lity to bind and lyse autologous tumor cells in C57BI/6 mice bearing t ransplanted melanoma B-16 was investigated. The ROI production by TAM and resident peritoneal macrophages (PM) from intact and tumor bearing mice was measured in luminol (LM) or lucigenin (LG)-enhanced chemilum inescent (CL) assay. We showed the low capacity of TAM to bind and lys e the autologous tumor cells. The amount of CL emitted by TAM in LG-sy stem was markedly low in comparison with CL of PM from health and espe cially tumor bearing mice.