Y. Yashiki et S. Yamashoji, EXTRACELLULAR REDUCTION OF MENADIONE AND FERRICYANIDE IN YEAST-CELL SUSPENSION, Journal of fermentation and bioengineering, 82(3), 1996, pp. 319-321
Extracellular reduction of menadione and ferricyanide were promoted by
glucose rather than ethanol in a yeast cell (Saccharomyces cerevisiae
IFO 2044) suspension. NAD(P)H: menadione oxidoreductase from yeast pl
asma membrane possessing ferricyanide reductase activity had higher su
bstrate specificity for NADH than for NADPH. Glucose produced a transi
ent increase in NADH content in the absence of menadione, but neither
glucose nor ethanol restored the NADH content upon menadione-catalyzed
ferricyanide reduction. These findings suggest that the NADH content
is controlled by the metabolism of glucose and ethanol, and that the e
xcess of NADH generated by glucose and ethanol is effectively consumed
by NADH:menadione oxidoreductase in the yeast transplasma membrane re
dox system.