Lmb. Garcia, BIOACTIVITY OF STORED LUTEINIZING-HORMONE-RELEASING HORMONE ANALOG (LHRHA) IN SEA BASS, LATES-CALCARIFER BLOCH, Journal of applied ichthyology, 12(2), 1996, pp. 91-93
The spawning induction activity of dissolved and pelleted (D-Ala(6), P
ro(9) N ethylamide) luteinizing hormone-releasing hormone analogue (LH
RHa) stored for various periods was assessed in mature female sea bass
. The spawning response of mature fish was reduced significantly after
injection of dissolved LHRHa (20 mu g kg(-1)) stored for more than 90
days in a refrigerator (4-10 degrees C) or for more than 30 days at r
oom temperature (28-30 degrees C). Similar to fish administered fresh
preparations of LHRHa, fish spawned successfully after injection of a
solution of LHRHa previously frozen, subjected to alternate freezing a
nd thawing, exposed to sunlight or implanted pelleted LHRHa (50 mu g k
g(-1)) stored at room temperature for 30-120 days. Loss of hormone bio
activity after prolonged storage may have been due to bacterial growth
in solubilized preparations. Injection or implantation of stored LHRH
a did not influence egg production among treated sea bass. These resul
ts demonstrated the relatively prolonged shelf life of stored LHRHa.