ATTEMPTS TO STIMULATE TROPHOBLAST CELLS I N-VITRO WITH PP14

Cytotrophoblast cells were isolated from human term placenta after ces arean section by fragmentation of villous tissue with trypsin and DNAs e I. Trophoblast cells fuse in vitro to syncytiotrophoblast cells and progesteron is released. Placental Protein 14 (PP14) was incubated (30 0 mu g/ml PP14 in 6 ml solution {6x10(6) cells}) with the cytotrophobl ast cells. Production of Progesteron is increased in PP14-trophoblast cell cultures compared to untreated trophoblast cells.