EFFECTS OF M(2) ANTAGONISTS ON IN-VIVO HIPPOCAMPAL ACETYLCHOLINE LEVELS

There is evidence that muscarinic receptors of the M(2) subtype are pr esynaptic autoreceptors that modify the release of acetylcholine (ACh) through a negative feedback mechanism. Blocking these receptors by se lective antagonists may therefore lead to increased ACh release, This in vivo microdialysis study examined the effects of three M(2) antagon ists, AF-DX 116, AF-DX 384, and AQ-RA 741, on hippocampal cholinergic neurotransmission. Drug (2, 4, 8, or 16 mu M) or vehicle (Ringer's sol ution) was perfused via a microdialysis probe into the CA1 hippocampal region of conscious male Fischer 344 rats, Levels of ACh and choline were assessed by HPLC-EC. When the dose was expressed in K-i multiples , all drugs (except AQ-RA 741 at the two highest concentrations) were found to be on the same linear dose-response curve. Choline levels wer e not affected by drug administration. All three compounds elevated AC h levels in a similar K-i-normalized dose-response fashion, strongly s upporting the concept that the proposed presynaptic mechanism of actio n is indeed based on the same M(2) receptor. Such elevations of ACh ma y not only improve performance on memory tasks, but may also have ther apeutic advantages in conditions of cholinergic hypofunction, such as Alzheimer's disease. Copyright (C) 1996 Elsevier Science Inc.