ALTERNATE SPLICE-SITE UTILIZATION IN THE GENE FOR THE CATALYTIC SUBUNIT OF THE DNA-ACTIVATED PROTEIN-KINASE, DNA-PKCS

Authors
CONNELLY MA ZHANG H KIELECZAWA J ANDERSON CW
Citation
Ma. Connelly et al., ALTERNATE SPLICE-SITE UTILIZATION IN THE GENE FOR THE CATALYTIC SUBUNIT OF THE DNA-ACTIVATED PROTEIN-KINASE, DNA-PKCS, Gene, 175(1-2), 1996, pp. 271-273
Citations number
17
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
175
Issue
1-2
Year of publication
1996
Pages
271 - 273
Database
ISI
SICI code
0378-1119(1996)175:1-2<271:ASUITG>2.0.ZU;2-M
Abstract
Analysis of polyA-selected RNAs and cDNA clones from several human cel l lines revealed the presence of a 93-bp exon in the PI kinase homolog y region of DNA-PKcs that was not present in the cDNA sequence derived from HeLa cell cDNA clones. RT-PCR showed that most DNA-PKcs mRNAs in human cells have this exon. Thus, the nascent DNA-PKcs, polypeptide i s composed of 4127 aa and has a predicted mel. wt. of 469 021 (470 kDa ).