CLONING AND CHARACTERIZATION OF DNAE, ENCODING THE CATALYTIC SUBUNIT OF REPLICATIVE DNA-POLYMERASE-III, FROM VIBRIO-CHOLERAE STRAIN C6706

Authors
FRANCO AA YEH PE JOHNSON JA BARRY EM GUERRA H MAURER R MORRIS JG
Citation
Aa. Franco et al., CLONING AND CHARACTERIZATION OF DNAE, ENCODING THE CATALYTIC SUBUNIT OF REPLICATIVE DNA-POLYMERASE-III, FROM VIBRIO-CHOLERAE STRAIN C6706, Gene, 175(1-2), 1996, pp. 281-283
Citations number
13
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
175
Issue
1-2
Year of publication
1996
Pages
281 - 283
Database
ISI
SICI code
0378-1119(1996)175:1-2<281:CACODE>2.0.ZU;2-J
Abstract
We report that Vibrio cholerae (Vc) contains a gene homologous to Esch erichia coli dnaE, the structural gene for the alpha (catalytic) subun it of replicative DNA polymerase III (PoIII). Despite 24% amino acid ( aa) differences in the encoded proteins, the Vc gene strongly compleme nts an E. coli dnaE temperature sensitive (ts) mutant, indicating that all functional features essential for replication are conserved.