A PROTOCOL FOR HORSEGRAM [MACROTYLOMA-UNIFLORUM (LAM) VERDC] CALLUS INDUCTION

Calli of Macrotyloma uniflorum (Lam.)Verdc.) Co.1 and Bgm.1 were estab lished from explants of epicotyl, hypocotyl, cotyledon, and young leaf on MS medium supplemented with auxins and cytokinins (2, 4-D, IAA, NA A, BAP, KN, and CM). When they were cultured subsequently on various c ombinations and different concentrations, BAP and 2,4-D were found to yield green compact callus in all explants. Yellow friable calli were obtained on MS medium supplemented with auxins (2,4-D or NAA or IAA). Browning of callus was observed at a higher concentration of (2.5 mgl( -1)) auxins after 25 days.