ENTRAPMENT OF COLISTIN INTO POLYHEXYLCYANOACRYLATE NANOPARTICLES - PREPARATION, DRUG-RELEASE AND TISSUE DISTRIBUTION IN MICE

The loading of colistin into polyhexylcyanoacrylate nanoparticles was performed with a high association rate. After freeze drying, the nanop article preparations showed high stability and the antibacterial activ ity of colistin was entirely maintained The release rate of colistin, along with the degradation of the polymer matrix, were studied in the presence of carboxyl-esterase. The in vivo distribution (liver, spleen , lungs, kidneys, serum and urine) of free and nanoparticles associate d colistin was investigated in mice after intravenous injection. The c olistin loaded nanoparticles were able to concentrate the antibiotic i n the organs of the reticuloendothelial system and especially in the l iver. The excretion of colistin by the kidneys was greatly reduced by the association with nanoparticles.