Cj. Liu et al., THE EFFECT OF GENOME AND SEX ON RECOMBINATION RATES IN PENNISETUM SPECIES, Theoretical and Applied Genetics, 93(5-6), 1996, pp. 902-908
The effects of homoeology and sex on recombination frequency were stud
ied in crosses between cultivated pearl millet, Pennisetum glaucum, an
d two wild subspecies, P. violaceum and P. mollissimum. For the two wi
ld x cultivated crosses, reciprocal three-way crosses were made betwee
n the F-1 hybrid and an inbred line (Tift 23DB(1)). The three-way cros
s populations were mapped to produce a female map of each wide cross (
where the F-1 was the female) and a male map (where the F-1 was the ma
le). Total genetic map lengths of the two inter-subspecies crosses wer
e broadly similar and around 85% of a comparable intervarietal map. In
the P. glaucum x P. mollissimum crosses, the map was further shortene
d by a large (40 cM) inversion in linkage group 1. Comparison of the r
ecovered recombinants from male and female meiocytes showed an overall
trend for the genetic maps to be longer in the male (similar to 10%)
in both inter-subspecific crosses; however, analysis of individual lin
kage intervals showed no significant differences. Gametophytic selecti
on was prevalent, and sometimes extreme, for example 12:1 in favour of
'wild' alleles in the P. glaucum x P. mollissimum male recombinant po
pulation. One of the loci which determines panicle type in cultivated
pearl millet and wild relatives, H, was mapped 9 cM from Xpsm812 on li
nkage group 7 in the P. violaceum cross.