K. Permaul et al., RANDOM-AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS SHOWS INTRASPECIES DIFFERENCES AMONG XANTHOMONAS ALBILINEANS STRAINS, Letters in applied microbiology, 23(5), 1996, pp. 307-311
Five strains of Xanthomonas albilineans, causal agent of leaf scald di
sease in sugarcane from various geographical regions, were compared us
ing random amplification of polymorphic DNA (RAPD) to determine whethe
r they could be differentiated at the DNA level. CsCl-purified genomic
DNA from these strains were amplified by the polymerase chain reactio
n (PCR) using arbitrary 10-mer primers according to standard RAPD cond
itions and the amplification product profiles analysed by conventional
agarose gel electrophoresis. Although most RAPD markers were common t
o all five strains, unique profiles for each strain were discernible u
sing four 10-mer arbitrary primers individually. Reproducible DNA fing
erprints indicate that RAPD analysis can be used to identify and diffe
rentiate the X. albilineans strains. This technique has the potential
for use in monitoring the appearance of foreign strains of X. albiline
ans in various geographical regions and could be used for the construc
tion of phylogenetic trees.