CHARACTERIZATION OF CRYPTOCOCCUS-NEOFORMANS BY RANDOM DNA AMPLIFICATION

Authors
RUMA P CHEN SCA SORRELL TC BROWNLEE AG
Citation
P. Ruma et al., CHARACTERIZATION OF CRYPTOCOCCUS-NEOFORMANS BY RANDOM DNA AMPLIFICATION, Letters in applied microbiology, 23(5), 1996, pp. 312-316
Citations number
29
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
23
Issue
5
Year of publication
1996
Pages
312 - 316
Database
ISI
SICI code
0266-8254(1996)23:5<312:COCBRD>2.0.ZU;2-E
Abstract
Random amplified polymorphic DNA (RAPD) was optimized and used to dist inguish between the varieties and serotypes of Cryptococcus neoformans . The RAPD technique distinguished between serotypes A, D or AD within C. neoformans var. neoformans, and revealed further differentiation w ithin each serotype. Four RAPD profiles were clearly recognizable with in C. neoformans var, gattii, although its two serotypes, B and C, wer e only differentiated with one primer combination out of seven.