BIODISTRIBUTION OF (111)INDIUM-LABELED ENGINEERED HUMAN-ANTIBODY CTMO1 IN OVARIAN-CANCER PATIENTS - INFLUENCE OF PROTEIN DOSE

Thirty-one patients suspected of having ovarian cancer received a sing le i.v. injection of radiolabeled (100 MBq In-111) engineered human CT MO1 (hCTMO1) to investigate its potential as an internalizing drug car rier. hCTMO1 is a complementary-determining region-grafted human IgG4 monoclonal antibody recognizing an ovarian carcinoma-associated antige n, the MUC-1-gene product. The amount of radioactivity was determined in tumor tissue, various normal tissues, including liver biopsies, and blood samples obtained at laparotomy, 6 days after injection of eithe r 0.1 or 1.0 mg hCTMO1/kg of body weight. Circulating antigen-15-3 was measurable in all patients before injection, and immune complex forma tion was already present at the end of infusion. In the 0.1 mg/kg grou p, most of the radioactivity was bound to immune complexes, whereas in the 1.0 mg/kg group, most was bound to IgG monomers. Increasing the h CTMO1 dose 10-fold did not influence the overall disappearance of In-1 11 from the blood, but the elimination half-life of (111)indium bound to immune complexes was increased 2-fold. Uptake in tumor tissue 6 day s postinjection at the 0.1 mg/kg dose was 7.6 times higher (P = 0.0009 ) than in normal tissue and 2.5 times higher (P = 0.03) than in blood. At the 1.0 mg/kg dose, the uptake in tumor tissue was 14.0 times high er (P = 0.0003) than in normal tissue and 8.1 times higher (P = 0.0007 ) than in blood. Liver activity was substantial (23.7 +/- 10.5 and 18. 3 +/- 6.7% of the injected dose/kg for the 0.1 and 1.0 mg/kg dose grou p, respectively). These results are superior to those found with other clinically tested anti-MUC-1 gene product antibodies. hCTMO1 seems to be a suitable carrier for cytotoxic agents in ovarian carcinoma patie nts; the better uptake results and tumor-to-blood ratios are obtained at the higher dose of 1.0 mg hCTMO1/kg body weight.