A 38-kDa lipoprotein of Treponema pallidum subsp. pallidum (T. pallidu
m), the syphilis spirochete, previously was identified as a putative h
omolog of E. coli MglB [Becker et al. (1994) Infect. Immun. 62, 1381-1
391]. In the present study, genome walking in regions adjacent to the
T. pallidum 38-kDa lipoprotein gene has identified three contiguous ge
nes (tp-mglB [formerly tpp38], tp-mglA, and tp-mglC) which appear to c
omprise a mgl-like operon in T. pallidum. A prominent transcript corre
sponding to tp-mglB, the first gene of the operon which encodes the ca
rbohydrate receptor, is synthesized by T. pallidum along with lesser a
bundant transcript(s) corresponding to the entire T. pallidum mgl oper
on. An active promoter 135 bp upstream of tp-mglB is believed to direc
t mRNA synthesis for the operon. This is the first membrane protein-en
coding operon of T. pallidum for which a putative function (glucose im
port) has been assigned. Furthermore, by analogy with E. coli MglB whi
ch interacts with the sensory transducer Trg to induce a chemotactic r
esponse, it is possible that T. pallidum also contains a homolog of E.
coli Trg or other methyl-accepting chemotaxis proteins. The existence
of a mgl operon in T. pallidum thus may have important implications w
ith respect to T. pallidum survival, tissue dissemination, and sensory
transduction during virulence expression.