SELECTIVE STAINING OF THE CEREBELLAR MOLECULAR LAYER BY SERUM IGG IN MILLER-FISHER AND RELATED SYNDROMES

During 1 year, we used immunocytochemical staining of human cerebellum to screen 1,488 serums for IgG autoantibodies to Hu and Yo antigens. Three serums had none of the classically described patterns of IgG bin ding but, instead, selectively stained the cerebellar molecular layer. Evaluation of clinical data showed that the patients had either typic al Miller Fisher syndrome (MFS) or Guillain-Barre syndrome with ophtha lmoplegia. Further analysis by ELISA assay showed that all three serum s had high titers of IgG anti-GQ1b autoantibodies. IgG autoantibody st aining of human cerebellum, which is used for the diagnosis of paraneo plastic disorders, may have additional specificity for other, presumab ly autoimmune, syndromes such as MFS. The specificity of the serum IgG autoantibody binding to the cerebellar molecular layer may be related to the ataxia that often occurs in these patients.