BIOLOGICAL APPLICATIONS OF ANTI-STOKES-RAMAN SPECTROSCOPY - QUANTITATIVE-ANALYSIS OF GLUCOSE IN PLASMA AND SERUM BY A HIGHLY SENSITIVE MULTICHANNEL RAMAN SPECTROMETER

This study demonstrates the potential of anti-Stokes Raman spectroscop y in investigating biological samples in a nondestructive manner; quan titative analysis of glucose in plasma and serum has been studied as a n example, The efficient collection of anti-Stokes Raman scattering by use of chromatic aberration of a lens has allowed us to obtain high-q uality anti-Stokes Raman spectra from glucose in plasma and serum, whi ch is a strongly fluorescent biological sample. The concentration of g lucose in these materials can be estimated by the anti-Stokes Raman in tensity of the band at 1130 cm(-1) due to the C-O stretching mode, The correlation coefficient between the concentration and the intensity h as been calculated to be 0.993 and 0.991 for glucose in plasma and ser um, respectively. The detection limits for these materials have been f ound to be 45 mg/dL and 50 mg/dL, respectively.