EVIDENCE FOR PROPEPTIDE-ASSISTED FOLDING OF THE CALCIUM-DEPENDENT PROTEASE OF THE CYANOBACTERIUM ANABAENA

Citation
K. Baier et al., EVIDENCE FOR PROPEPTIDE-ASSISTED FOLDING OF THE CALCIUM-DEPENDENT PROTEASE OF THE CYANOBACTERIUM ANABAENA, European journal of biochemistry, 241(3), 1996, pp. 750-755
Citations number
24
Categorie Soggetti
Biology
ISSN journal
00142956
Volume
241
Issue
3
Year of publication
1996
Pages
750 - 755
Database
ISI
SICI code
0014-2956(1996)241:3<750:EFPFOT>2.0.ZU;2-G
Abstract
The Ca2+-dependent protease of the cyanobacterium Anabaena variabilis is a cytoplasmic enzyme with a substrate specificity like trypsin. Its previously published DNA sequence [Maldener, I., Lockau, W., Cai, Y. & Wolk, C. P. (1991) Mol. Gen. Genet. 225, 113-120] contained a sequen cing error. Here report the corrected sequence which shows, that the C a2+-protease belongs to the family of subtilases (subtilisin-like seri ne proteases). Consistent with its cytoplasmic localization. a pre-seq uence is not found. The enzyme is produced as a precursor with a large amino-terminal propeptide. Expression of the pro-region and mature re gion (protease domain) in Escherichia coli cells in trans demonstrates that formation of the active enzyme requires the propeptide. The resu lts demonstrate that propeptide-assisted protein folding also occurs w ith cytoplasmic enzymes, in support of the hypothesis that this mechan ism is a widespread phenomenon.