SENSITIVITY OF DAUDI CELLS TO TUMOR-NECROSIS-FACTOR-ALPHA - EARLY CHANGES IN POLYPHOSPHOINOSITIDE METABOLISM

The effects of recombinant Tumor Necrosis Factor alpha (r-TNF alpha) o n polyphosphoinositide metabolism were examined in a Burkitt Lymphoma cell line (Daudi cells). After 1 h of in vitro treatment with r-TNF al pha, the incorporation of (32)Pi into phosphatidylinositol 4,5-phospha te (PtdInsP(2)), phosphatidylinositol 4-phosphate (PtdInsP) and phosph atidylinositol (PtdIns) was reduced compared with controls, confirming previous findings observed in other cell lines of a specific PtdIns b reakdown following r-TNF alpha treatment. The novelty of this study is therefore the demonstration of early changes in polyphosphoinositide metabolism during the antiproliferative response elicited by this cyto kine in Daudi cells. (C) 1996 Academic Press Limited