VALIDATION OF A RIBOSOMAL DNA-POLYMERASE CHAIN-REACTION SPECIES DIAGNOSTIC ASSAY FOR THE COMMON MALARIA MOSQUITO (DIPTERA, CULICIDAE) SIBLING SPECIES COMPLEX

Authors
RUTLEDGE CR CORNEL AJ MEEK CL COLLINS FH
Citation
Cr. Rutledge et al., VALIDATION OF A RIBOSOMAL DNA-POLYMERASE CHAIN-REACTION SPECIES DIAGNOSTIC ASSAY FOR THE COMMON MALARIA MOSQUITO (DIPTERA, CULICIDAE) SIBLING SPECIES COMPLEX, Journal of medical entomology, 33(6), 1996, pp. 952-954
Citations number
11
Categorie Soggetti
Entomology
Journal title
Journal of medical entomology → ACNP
ISSN journal
00222585
Volume
33
Issue
6
Year of publication
1996
Pages
952 - 954
Database
ISI
SICI code
0022-2585(1996)33:6<952:VOARDC>2.0.ZU;2-8
Abstract
A polymerase chain reaction method for identifying individuals in the Anopheles quadrimaculatus Say sibling species complex was validated fo r wild mosquitoes from Louisiana and Mississippi. This method distingu ished An. quadrimaculatus species A, B, C, and D by detecting species- specific differences in the 2nd internal transcribed spacer of ribosom al DNA and was 100% specific and 95% sensitive.