VASOPRESSIN AND RENIN-ANGIOTENSIN MAINTAIN ARTERIAL-PRESSURE DURING PEEP IN NONEXPANDED, CONSCIOUS DOGS

Increases of plasma arginine vasopressin (AVP) and plasma renin activi ty (PRA) during controlled mechanical ventilation (CMV) with positive end-expiratory pressure (PEEP) induce positive fluid balances by decre asing renal excretion. We investigated whether elevated levels of AVP and/or PRA maintain mean arterial pressure (MAP) during PEEP under con ditions where plasma volume is not expanded. Six conscious chronically tracheotomized beagle dogs, kept under standardized conditions, were investigated in four protocols. They were 1) control: 1 h spontaneous breathing with a continuous positive airway pressure of 4 cmH(2)O (CPA P 4) followed by 2 h CMV with PEEP, resulting in a mean airway pressur e of similar to 20 cmH(2)O (%MV 20 referred to as ''PEEP''); 2)vasopre ssin blockade: Ih CPAP 4, 2 h PEEP after intravenous application of an AVP V-1-receptor antagonist (AVPA); 3) converting enzyme inhibition: 1 h CPAP 4, 2 h PEEP plus angiotensin-converting enzyme inhibition (AC EI); and 4) combined blockade: 1 h CPAP 4, 2 h PEEP plus AVPA + ACEI. In AVPA + ACEI, MAP decreased during PEEP from 101 +/- 4 to 75 +/- 10 mmHg, glomerular filtration rate (GFR) decreased from 3.6 +/- 0.3 to 1 .7 +/- 0.7 ml . min(-1). kg body wt(-1), heart rate increased from 95 +/- 10 to 122 +/- 7 beats/min, plasma aldosterone increased from 62 +/ - 26 to 353 +/- 63 pg/ml, plasma epinephrine increased from 81 +/- 15 to 352 +/- 89 pg/ml (all changes P < 0.05), and plasma norepinephrine did not change. Neither MAP nor GFR changed during PEEP in control exp eriments in which both PRA and AVP increased, in AVPA experiments in w hich PRA increased, or in ACEI experiments in which AVP increased. We conclude that both AVP and angiotensin II contribute to the maintenanc e of MAP and GFR during PEEP. When both hormones are inhibited, no imm ediate compensation exists to prevent an acute fall in MAP and GFR.