CHANGES IN T-LYMPHOCYTE SUBPOPULATIONS AND SERUM LYMPHOKINE CONCENTRATIONS AFTER PARTIAL-HEPATECTOMY IN RATS

Citation
Hs. Lai et al., CHANGES IN T-LYMPHOCYTE SUBPOPULATIONS AND SERUM LYMPHOKINE CONCENTRATIONS AFTER PARTIAL-HEPATECTOMY IN RATS, Nutrition, 12(10), 1996, pp. 700-705
Citations number
32
Categorie Soggetti
Nutrition & Dietetics
Journal title
Nutrition
ISSN journal
08999007 → ACNP
Volume
12
Issue
10
Year of publication
1996
Pages
700 - 705
Database
ISI
SICI code
0899-9007(1996)12:10<700:CITSAS>2.0.ZU;2-Q
Abstract
The relationship between liver regeneration and immunological response does exist. Extrathymic T cells and serum lymphokines are believed to be changed after partial hepatectomy, but there is still much controv ersy. This study evaluates the changes of peripheral blood T-lymphocyt e subpopulations, natural killer (NK) cells, serum lymphokines, and NK cells in the remnant liver after partial hepatectomy. Male Wistar rat s weighing around 200 g were used as subjects. Partial hepatectomies ( 67%) were done. Counting T-lymphocyte subpopulations (includes T3, T4, T8, T-IL2R, and NK cells) by using fluorescin isothiocyanate or phyco erythrin was performed preoperatively and on 1, 2, 3, 5, 7, and 10 d p ostoperatively. Serum gamma-interferon (gamma-IFN), interleukin-2 and interleukin-2 receptor concentrations were measured. Absolute number o f NK cells in the remnant liver was also counted. Rats with right-side nephrectomy, sham operation, and no operation were used as control gr oups, Comparing the difference between all groups, we found that all T -lymphocyte subpopulations in peripheral blood, serum gamma-IFN, perip heral blood NK cells, and the absolute number of NK cells in the remna nt liver of hepatectomized rats increased markedly, and were significa ntly higher than control groups rats, on the fifth and seventh post-he patectomy day. It is suggested that gamma-IFN and NK cells were activa ted markedly and might play important roles in the regulation of liver regeneration after partial hepatectomy.