P. Gouet et al., FERRYL INTERMEDIATES OF CATALASE CAPTURED BY TIME-RESOLVED WEISSENBERG CRYSTALLOGRAPHY AND UV-VIS SPECTROSCOPY, Nature structural biology, 3(11), 1996, pp. 951-956
Various enzymes use semi-stable ferryl intermediates and free radicals
during their catalytic cycle, amongst them haem catalases. Structures
for two transient intermediates (compounds I and II) of the NADPH-dep
endent catalase from Proteus mirabilis (PMC) have been determined by t
ime-resolved X-ray crystallography and single crystal microspectrophot
ometry. The results show the formation and transformation of the ferry
l group in the haem, and the unexpected binding of an anion during thi
s reaction at a site distant from the haem.