FERRYL INTERMEDIATES OF CATALASE CAPTURED BY TIME-RESOLVED WEISSENBERG CRYSTALLOGRAPHY AND UV-VIS SPECTROSCOPY

Authors
GOUET P JOUVE HM WILLIAMS PA ANDERSSON I ANDREOLETTI P NUSSAUME L HAJDU J
Citation
P. Gouet et al., FERRYL INTERMEDIATES OF CATALASE CAPTURED BY TIME-RESOLVED WEISSENBERG CRYSTALLOGRAPHY AND UV-VIS SPECTROSCOPY, Nature structural biology, 3(11), 1996, pp. 951-956
Citations number
36
Categorie Soggetti
Biology,"Cell Biology
Journal title
Nature structural biology → ACNP
ISSN journal
10728368
Volume
3
Issue
11
Year of publication
1996
Pages
951 - 956
Database
ISI
SICI code
1072-8368(1996)3:11<951:FIOCCB>2.0.ZU;2-P
Abstract
Various enzymes use semi-stable ferryl intermediates and free radicals during their catalytic cycle, amongst them haem catalases. Structures for two transient intermediates (compounds I and II) of the NADPH-dep endent catalase from Proteus mirabilis (PMC) have been determined by t ime-resolved X-ray crystallography and single crystal microspectrophot ometry. The results show the formation and transformation of the ferry l group in the haem, and the unexpected binding of an anion during thi s reaction at a site distant from the haem.