A 3-HYBRID SYSTEM FOR DETECTING SMALL LIGAND-PROTEIN RECEPTOR INTERACTIONS

Small ligand-receptor interactions underlie many fundamental processes in biology and form the basis for pharmacological intervention of hum an diseases in medicine. We report herein a genetic system, named the yeast three-hybrid system, for detecting ligand-receptor interactions in vivo. This system is adapted from the yeast two-hybrid system with which a third synthetic hybrid ligand is combined. The feasibility of this system was demonstrated using as the hybrid ligand a heterodimer of covalently linked dexamethasone and FK506. Yeast expressing fusion proteins of the hormone binding domain of the rat glucocorticoid recep tor fused to the LexA DNA-binding domain and of FKBP12 fused to a tran scriptional activation domain activated reporter genes when plated on medium containing the dexamethasone-FK506 heterodimer. The reporter ge ne activation is completely abrogated in a competitive manner by the p resence of excess FK506. Using this system, we screened a Jurkat cDNA library fused to the transcriptional activation domain in yeast expres sing the hormone binding domain of rat glucocorticoid receptor-LexA DN A binding domain fusion protein in the presence of dexamethasone-FK506 heterodimer. We isolated overlapping clones of human FKBP12. These re sults demonstrate that the three-hybrid system can be used to discover receptors for small ligands and to screen for new ligands to known re ceptors.