QUANTITATIVE-ANALYSIS OF METABOLITES IN URINE USING A HIGHLY PRECISE,COMPACT NEAR-INFRARED RAMAN SPECTROMETER

This paper demonstrates the potential of a newly devised near-infrared Raman system in quantitative analysis of metabolites in urine. By usi ng the system, Raman spectra have been measured for urine to which glu cose, acetone, urea, or creatine was added artificially, and for urine including the four components simultaneously. The concentrations of g lucose, acetone, urea, and creatine in urine which includes one partic ular component artificially have been determined by the intensities of Raman bands at 1130, 790, 1013, and 692 cm(-1), respectively. The cor relation coefficient (R) between the concentration of glucose, acetone , urea, or creatine and the Raman intensity has been calculated to be 0.981, 0.992, 0.991, and 0.998, respectively. Their detection limits h ave been found to be 41 mg/dl, 40 mg/dl, 4.9 mg/ml, and 1.5 mg/ml, res pectively. The concentrations of glucose, acetone, urea, and creatine in urine including the four components simultaneously have also been d etermined by the intensities of the four bands. Again, good correlatio ns were obtained between the concentrations and the Raman intensities with the R values of 0.924 (glucose), 0.987 (acetone), 0.962 (urea), a nd 0.923 (creatine), respectively.