DEVELOPMENT OF A PYRROLOQUINOLINE QUINONE (PQQ) MEDIATED GLUCOSE-OXIDASE ENZYME ELECTRODE FOR DETECTION OF GLUCOSE IN FRUIT JUICE

Authors
LOUGHRAN MG HALL JM TURNER APF
Citation
Mg. Loughran et al., DEVELOPMENT OF A PYRROLOQUINOLINE QUINONE (PQQ) MEDIATED GLUCOSE-OXIDASE ENZYME ELECTRODE FOR DETECTION OF GLUCOSE IN FRUIT JUICE, Electroanalysis, 8(10), 1996, pp. 870-875
Citations number
27
Categorie Soggetti
Chemistry Analytical
Journal title
Electroanalysis → ACNP
ISSN journal
10400397
Volume
8
Issue
10
Year of publication
1996
Pages
870 - 875
Database
ISI
SICI code
1040-0397(1996)8:10<870:DOAPQ(>2.0.ZU;2-D
Abstract
The electrochemistry of pyrroloquinoline quinone (PQQ) was examined an d the coupling of PQQ with glucose oxidase was studied at different pH values. Optimal interaction between PQQ and glucose oxidase was obser ved at pH 3.5. Results from these studies enabled construction of PQQ- glucose oxidase-modified enzyme electrodes for the detection of glucos e in Ribena blackcurrant juice. The corresponding amperometric determi nation of glucose was in good agreement with results obtained using co mmercial enzymatic test kits supplied by Boehringer Mannheim and Sigma Diagnostics.